The building is located on İkiçeşmelik Street. Bikur Holim Synagogue was built in 1724 by Salamon de Ciaves, a Dutch-born Jew from Izmir. The houses and shops adjacent to the building were financially attached to the synagogue to generate income. Single storey additional buildings in front of the main building separate the synagogue from Ikiçesmelik Street. It was rebuilt by Manuel de Ciavas after being burned in a fire in 1772. Today, the building is being restored by a contractor firm with the allowance provided by the Jewish community. The unstable roof of the building was repaired during the restoration works. The synagogue which cannot be used today will be opened to use at the end of the restoration works. When the decision to expand the street in the zoning plan for İkiçeşmelik is applied, the single storey annexes in front of the building will also be eliminated.

Since the synagogue is a 17th century structure, it is a Sephardic synagogue and was built with a central plan. Rebuilt for the second time by Manuel de Ciaves, the building is original with everything except its paints. The main site of the synagogue is reached from an entrance site as in the Talmud Tora synagogue. The upper part of this entrance is mehizah. The entrance space is arranged as a front synagogue space, as in the Talmud Tora synagogue and Etz Hayim synagogue. The main space has the height of two floors and is structured as single volume.

Some of the synagogues in the Ottoman cities had prisons belonging to community in their basements. The basement of Bikur Holim Synagogue is thought to be used for this purpose. On the porch of the Torah cupboard there are geometric decorations resembling Greek architecture. Bikur Holim Synagogue has rich ornaments and interior decorations.